A Stereological Study on Hippocampal Subfields Following Administration of Methamphetamine in Male Mice
Authors
Abstract:
Background and Aims: This study examined sub-chronic effects of Methamphetamine (METH) on the stereological parameters in the Cornu Ammonis (CA) of the hippocampus in adult mice. Materials and Methods: Fifteen adult male mice, eight weeks old, were randomly divided into three groups: receive saline (controls), or low-dose (LD) 2.5 mg/kg METH, or high-dose (HD) 25 mg/kg METH, via daily intraperitoneal administrations for one month. The Cavalieri principle was used to estimate the volume of CA hippocampal field. The physical disector was used to determine the numerical density of CA1 and CA3 pyramidal cells. The qualitative Golgi staining was also done for analyzing the dendritic morphology of CA1 and CA3 pyramidal cells. Results: Our results showed that in METH-treated mice, the volumes of the Oreins, Pyramidal and Radiatum-Lacunosum-Moleculare layers in the CA1 and CA3, the entire volumes of the CA1 and CA3 subfields and the entire hippocampal volume were significantly increased compared to the control animals (p˂0.001). The estimated total number and numerical density of pyramidal cells in the CA1 and CA3 in both treated groups showed a significant decrease in comparison with the controls (p˂0.001). The pyramidal neurons of CA1 and CA3 in treated mice had more dendritic arborization and greater dendritic length than control mice. Conclusions: Our findings indicate that sub-chronic METH injection induces stereological changes in the structure of the hippocampus in adult mice. A neuroanatomical basis may be related to the primarily reported impairment of learning and memory abilities in the METH user.
similar resources
a stereological study on hippocampal subfields following administration of methamphetamine in male mice
background and aims: this study examined sub-chronic effects of methamphetamine (meth) on the stereological parameters in the cornu ammonis (ca) of the hippocampus in adult mice. materials and methods: fifteen adult male mice, eight weeks old, were randomly divided into three groups: receive saline (controls), or low-dose (ld) 2.5 mg/kg meth, or high-dose (hd) 25 mg/kg meth, via daily intraperi...
full textStudy on The Reproductive Organs and Fertility of The Male Mice following Administration of Metronidazole
full text
A Morphometeric Study on CA3 Hippocampal Field in Young Rats Following Maternal Administration of Boswellia Serrata Resin During Gestation
Objective It has previously been shown that prenatal maternal administration of Boswellia serrata gum resin (Frankincense) improved learning and memory performance associated with an increase in the size of neuronal bodies in CA3 (Cornu Ammonis) of hippocampus. Continuing the previous work, a morphometric study was designed on CA3 field to examine precisely the effect of prenatal administratio...
full textreflections on taught courses of the iranian ma program in english translation: a mixed-methods study
the issue of curriculum and syllabus evaluation and revision has been in center of attention right from when curriculum came into attention of educational institutions. thus everywhere in the world in educational institutions curricula and syllabi are evaluated and revised based on the goals, the needs, existing content, etc.. in iran any curriculum is designed in a committee of specialists and...
Study of Volumetric Changes of Rats Brain Following Lithium Administration by Means of Stereological Methods
Purpose: Lithium is an alkali metal, which has many industrial and medical uses. These include cluster and migraine headaches, mood disorder, schizophrenia and it also is important in treatment of bipolar disorder. In this study, effects of Lithium on volumetric parameters of cerebrum were investigated by using stereological methods. Materials and Methods: Twenty mature Wistar male rats (Age: ...
full textMy Resources
Journal title
volume 3 issue 4
pages 270- 281
publication date 2016-11
By following a journal you will be notified via email when a new issue of this journal is published.
No Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023